Protein-drug Binding Assays

The reader is referred to other reviews for detailed discussions of the electronic states and luminescence of nucleic acids and their constituents/0 fluorescence correlation spectroscopy/2) spectroscopy of dye/DNA complexes/0 and ethidium fluorescence assays/4,0 A brief review of early work on DNA dynamics as well as a review of tRNA kinetics and dynamics have also appeared. The diverse and voluminous literature on the use of fluorescence techniques to assay the binding of proteins and antitumor drugs to nucleic acids and on the use of fluorescent DNA/dye complexes in cytometry and cytochemistry lies entirely outside the scope of this chapter. 

Fig. 2.26 Plot of the DBQ (Drug, D)/CMVP A144L (Protein, P) molar ratios for the incubated concentrations as a function of the non-covalently bound concentrations as determined in the titration study of the GPC spin column eluates assayed by ESI-MS (see Table 2.5). The shape of the curve indicates that up to three drugs bind non-covalently and non-specifically to CMVP A144L.

Y Ishihama, T Miwa, N Asakawa. Drug-plasma protein binding assay by electrokinetic chromatography-frontal analysis. Electrophoresis 23 951-955... 

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