Droplet size measurement coulter counter

Figure 6 Oil droplet size distribution of on olive oil emulsion, stabilized with hydroxy-propyl mcthylceUkiloae, after different emulsification procedures blender (triangles), ultrasonic probe (squares), and ultrasonic homogenize (stars). Theoretical distributions were calculated from Coulter Counter measurements using a software program, assuming spherical particles.

A fourth way is to count droplets individually. First, one has to dilute the emulsion strongly. Then, this diluted emulsion is pushed through a small hole. At the same time, the electrical conductivity through the hole is measured. Every time an emulsion droplet moves through the hole, the droplet will obscure part of the hole, which suddenly reduces the conductivity through the hole— the larger the droplet is, the stronger is the effect. Also in this way a droplet size distribution can be obtained. This method is usually referred to as the Coulter counter method, after an important manufacturer of this type of equipment. 

Measurement of the PIT can at best be used as a guide for the preparation of stable emulsions. Any assessments of stability should be evaluated by following the droplet size distribution as a function of time, using a Coulter counter or... 

The break-up of Newtonian liquids with various viscosities t] can be investigated by mastication of small oil samples and measuring the resulting droplet size distribution, using a Coulter Counter or a Master sizer. Samples are expectorated into a suitable surfactant solution, e.g. Tween (to prevent coalescence during measurements). can be measured at 37 °C (body temperature) using a suitable rheome-... 

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