Diuretics sports, abuse

The use of reaction sequences is also common in the analysis of pharmaceuticals. For example, for the purity test for hydrochlorothiazide (HCT) the DAB specifies a nitration reaction followed by reaction with naphthylethylenediamine dihydrochloride, in which the reaction sequence is performed as in the example given below. HCT is a diuretic which is often used in combination with beta-blockers for the treatment of hypertension (high blood pressure). Diuretics of this type are also abused in sport (doping) and appear on the list of banned substances prepared by the IOC (International Olympic Committee). In the quantitative determination of HCT in urine, the red azo dyes formed in the derivatization (parent substance and metabohtes) is regarded as a doping-positive result [111]. The complete specification for this analytical method, known as Application A-43.2, can be obtained from CAM AG. 

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