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Distortions, light microscopy

Another source of distortion that must be taken into account in optical sectioning microscopy is the geometric distortion generated by focusing light through layers with different refractive indices (Fig. 5). [Pg.209]

The limiting spatial resolution of confocal microscopy is demonstrated in Fig. 8.12a with the help of objects that have diameters far below the wavelength of the imaging light here, fluorescing spheres of 110 nm diameter. The lateral resolution is of the order of a few hundred nanometers, while the vertical resolution of the order of a micrometer. This latter distortion of the spheres towards elongated objects results from the fact that the axial resolution is a factor of three to four smaller than the focal resolution. [Pg.208]

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See also in sourсe #XX -- [ Pg.1063 ]



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Light microscopy

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