Disc electrophoresis apparatus

Another way of dealing with an excised zone is described by Adesnik (1970), and has been adapted to strips of gel cut from broad slabs on which are run as much as 5 mg of RNA (Adams et al. 1969). The gel slice is coarsely chopped up and placed into a cylindrical tube 10 x 1 cm, one end of which is covered with DEAE paper, and over this a layer of cellophane attached to the tube with a rubber band. The tube is filled with buffer (0.04 M Tris acetate, pH 8.3) and placed in a disc electrophoresis apparatus. After electrophoresis the RNA is found trapped in the ion-exchange paper, from which it is eluted as before. 

Figure 29-2, p. 346, shows a commercial disc electrophoresis apparatus. 

Figure 29-2. A commercial disc electrophoresis apparatus. (Courtesy - Buchler Instruments, Inc., Ft. Lee, NJ)...

The disc electrophoresis apparatus is the same as that described on p. 346. 

Apparatus, disc electrophoresis 1 Aspirator, water 1 Beaker, 100 mL 1 Beaker, 600 mL 1 Board, 5 cm x 20 cm x 6 mm 1 Bottles, wash, 250 mL 1 Brush, test tube, small... 

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