Disadvantages of Fluorescence and Phosphorescence

Other compounds that fluoresce may need to be removed from the system if the spectra overlap. This can be done, for example, by column chromatography. Peaks may appear in the fluorescence spectrum, which are due to other emission and scattering processes Rayleigh, TyndaU, and Raman scattering may be seen because of the high intensity of the light source used. Peaks due to fluorescent impurities may occur. 

Photochanical decomposition or photochanical reaction may be induced by the intense light sources used. In general, the approach of using the longest excitation wavelength possible and the shortest measurement time possible will minimize this problem. 

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