Diphosphopyridine nucleotide, isolation

The toxic action of bilirubin on tissue metabolism has been demonstrated both by in vitro and in vivo experiments. Lathe (LI) added bilirubin to brain brei, in concentrations such as occur in hemolytic disease of the newborn and prematurity, and observed a decrease in oxygen consumption. Day (D3) was able to reverse this depressed respiration by the addition of cytochrome c or methylene blue. Bowen and Waters (B16) then showed that this reversal could also be efFected by diphosphopyridine nucleotide. Zetterstrom and Ernster (Zl), using isolated rat liver mitochondria, showed that bilirubin in concentrations... 

Ueda T, Lode ET, Coon MJ. 1972. Enzymatic omega-oxidation, 6 isolation of homogeneous reduced diphosphopyridine nucleotide-rubredoxin reductase. J Biol Chem 247 2109-2116. 

Fia. 7. E feot of daily treatment with amethopterin on the incorporation of for-mate-C into the diphosphopyridine nucleotide of mouse liver. Mice received daily subcutaneous injections of amethopterin for 7 days. On the eighth day they were injected with 600 mg/kg of nicotinamide and fonnate-C After 6 hr the livers were homogenized with triohloroacetio acid and the diphosphopyridine nucleotide was isolated by ion-exchange chromatography. From Shuster et dl. QSM). 

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