1.2- Dioxetanes molecular biology

C. Dioxetane Substrates for Molecular Biology and Clinical Assays. 1198... 

Beck S, Koster H. Applications of dioxetane chemiluminescent probes to molecular biology. Anal Chem 2000 45 2258-70. 

The literature on organic chemistry abounds with examples of molecules that, in appropriate conditions, will exhibit chemiluminescence (Al, Ml7, M22). Nevertheless, the development of chemiluminescence as a diagnostic tool in immunology, clinical chemistry, and molecular biology has resulted almost entirely from the use of three or four compounds and their respective derivatives. There are several reasons for this dichotomy. First, many chemiluminescent compounds are inherently unstable and difficult to control. Conversely, the light-producing reactions of extremely stable compounds (such as some of the dioxetanes) may be so... 

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