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1.2- Dioxetanes bioassays

A. Firefly Blolumlnescence as a Prototype for a Dioxetane-based Triggered Chemiluminescence (CIEEL) In Bioassays... [Pg.1191]

Dioxetanes, labeled with triggers sensitive to the alkaline-phosphatase enzyme, serve as highly sensitive chemiluminescent probes in numerous bioassays. Current applications include immunoassays, membrane-based detection of proteins and nucleic acids, and microplate-based and array-based nucleic-acid detection. ... [Pg.1198]

CIEEL is of particular interest for the development of modern chemiluminescent bioassays. The most popular clinical bioassays utilize thermally persistent spiro-adamantyl-substituted dioxetanes with a protected phenolate moiety. These designed 1,2-dioxetanes include an energy source, a fluorophore, and a trigger grouping, and are therefore structurally similar to bioluminescent substrates such as firefly luciferin. Three main commercial dioxetanes 75 are available as one-reagent assays for alkaline phosphatase and are sold under the name of AMPPD (R1 = R2 = H), CSPD (R1 = Cl, R2 = H), and CDP-Star (R1 = R2 = Cl) <2006S1781, 2003ANA279>. These substrates are sensitive to 10 21 mol of alkaline phosphatase in solution. [Pg.792]

See other pages where 1.2- Dioxetanes bioassays is mentioned: [Pg.1173]    [Pg.1182]    [Pg.1184]    [Pg.1192]    [Pg.1193]    [Pg.1198]    [Pg.1200]    [Pg.1205]    [Pg.1449]    [Pg.1173]    [Pg.1184]    [Pg.1192]    [Pg.1193]    [Pg.1200]    [Pg.1205]    [Pg.776]    [Pg.197]   
See also in sourсe #XX -- [ Pg.1173 ]



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