2,4-Dinitrofluorobenzene Sanger

Dinitrofluorobenzene (Sanger s reagent) [70-34-8] M 186.1, m 25-27 , b 133 /2mm, 140-141 /5mm, d 1.483. Crystd from ether or EtOH. Vacuum distd through a Todd Column (see p. 174). If it is to be purified by distn in vacuo, the distn unit must be allowed to cool before air is allowed into the apparatus otherwise the residue carbonises spontaneously and an EXPLOSION may occur. The material is a skin irritant and may cause serious dermatitis. 

A method for determining the N-terminal amino acid of a peptide. The peptide is treated with 2,4-dinitrofluorobenzene (Sanger s reagent), then completely hydrolyzed. The derivatized amino acid is easily identified, but the rest of the peptide is destroyed in the hydrolysis, (p. 1180)... 

Determination of N-terminal amino acid residues by use of 2,4-dinitrofluorobenzene (Sanger s reagent). 

Solution 5-dimethylaminonaphthalenesulfonyl chloride (dansyl chloride) reacts with an amino acid in the same manner as does 2,4 dinitrofluorobenzene (Sanger s reagent) it bonds with the unprotected a-amino group. 

An alkylator which has found use in analytical work (sequencing of proteins the determination of the sequence in which the amino acid monomers are arranged in the protein polymer) is 2,4-dinitrofluorobenzene Sanger s reagent). 

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