Dihydrogen cations chemical bonding

The chromatographic procedure may be carried out using (a) two stainless steel columns in series the first (25 cm x 4.6 mm) packed with particles of silica, the surface of which has been modified with chemically bonded hexysilyl groups (5 /im) (Spherisorb C6 is suitable) and the second (25 cm x 4.6 mm) packed with cation exchange resin (10 /an) (Partisil-10 SCX is suitable) (b) as the mobile phase at a flow rate of 1.0 ml/min, a mixture of 25 volumes of acetonitrile, 25 volumes of a phosphate buffer solution prepared by dissolving 58.5 g of sodium dihydrogen orthophosphate... 

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