3,3-Dihexyloxacarbocyanine iodide

The lysolecithin treatment removes all or most of the plasma membrane and NE from the sperm, but a small amount of material can sometimes be seen at the ends of the sperm heads when they are stained with the membrane dye 3,3 -dihexyloxacarbocyanine iodide (DiOQ cat. 14414 Eastman Kodak Co., Rochester, NY). However, most of the surface of the sperm chromatin is devoid of any membrane. Centrioles and even short pieces of flagella frequently remain attached to the nuclei. Therefore, technically the structures that are isolated by this procedure are permeabilized sperm rather than sperm nuclei. 

Duckett, J. G. Read, D. J. The use of the fluorescent dye, 3,3 -dihexyloxacarbocyanine iodide, for selective staining of ascomycete fungi associated with liverwort rhizoids and ericoid mycorrhizal roots. New Phytol. 1991, 118, 259-272. 

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