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Digoxigenin random primed

In addition to biotin, a digoxigenylated derivative of dUTP was also synthesized. This derivative of dUTP can be incorporated into DNA by Pol I (or the Klenow fragment of Pol I). Therefore, digoxigenin-labeled DNA probes can be prepared by nick translation or random primed-labeling methods developed for the biotin system. It is almost certain that more nonradioactive alternatives to biotin and digoxigenin will be developed in the future. Chemiluminescent methods for nonradioactive probe detection are now widely being used... [Pg.379]

The probe DNA is labeled by incorporation of the thymidine analog digoxigenin-11-dUTP by the random primed labeling technique of Feinberg Vogelstein (1983). [Pg.321]

Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming... [Pg.73]

See other pages where Digoxigenin random primed is mentioned: [Pg.289]    [Pg.289]    [Pg.216]    [Pg.82]    [Pg.276]    [Pg.75]    [Pg.177]    [Pg.559]    [Pg.8]   
See also in sourсe #XX -- [ Pg.73 , Pg.74 ]



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Random priming

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