Differential imaging

Our next step will be to use differential imaging to study cluster chemistry and examine the effects of solvation (hydration) on electronic structure ... 

FIGURE 9.14 Illustration of multi-dimensional differential imaging using HGIO CARS excitation. (a) Schematic of the sample configuration (b) xz cross-section of the glass/DMSO/ deuterated-dodecane/glass interface. 

Krishnamachari, V. V., and Potma, E. O. 2008. Multi-dimensional differential imaging with FE-CARS microscopy. Vib. Spectmsc. DOI 10.1016/j.vibspec.2008.07.009. 

Liu, C., and Kim, D. Y. 2007. Differential imaging in coherent anti-Stokes Raman scattering microscopy with Laguerre-Gaussian excitation beams. Opt. Express 15 10123-34. 

D-differential imaging gel electrophoresis (DIGE) is also a gel-based procedure, but one that is more precise than the 2-DE approach. A key element of this procedure is labeling of proteins in the two experimental samples with different colored amine reactive fluorescent dyes.46 47 A common procedure is to label the control sample with Cy-5 cyanine dye and the test sample with Cy-3 cyanine dye. These two dyes display fluorescence at 670 and 570 nm, respectively. The two samples are then mixed in equal concentrations and separated... 

Blasdel GG. 1992. Differential imaging of ocular dominance and orientation selectivity in monkey striate cortex. J Neurosci 12 3115-3138. 

FIGURE 12.7 (See color insert following page 210.) Differential imaging analysis of a control vs. a gentamicin-treated rat kidney section. Average spectrum (cortex) and intensity distribution of m/z 12,959 on a control and a treated kidney section. (Adapted from Meistermann, H. et al., Mol. Cell. Proteomics, 5, 1876, 2006. With permission.)... 

Scharfetter, H., Brunner, R, Merwa, R., 2006a. Magnetic induction tomography single-step solution of the 3-D inverse problem for differential image reconstruction. Int. J. Inf. Syst. Sci. 2 (4), 585—606. 

Quantification by Two-Dimensional Differential Imaging Gel Electrophoresis 2D-DIGE is a more precise version of the 2-DE approach. This method uses multicolored cyanine dyes to label proteins in the two experimental samples... 

Fig. 25.8. Differential images of the distribution of peptides with simiiar moiecuiar masses (peptide ion images extracted at m/z 0.0025 Da). Note that these images demonstrate the distributions of singie isotopes i.e., the 1,011.410 moiecuiar species is clearly resolved from the second isotopes of 1,010.472 and 1,010.590 (respectively 1,011.472 and 1,011.590) (see Note 7). The higher relative intensity of the 1,011.410 signal vs. the 1,010.472 signal is in good agreement with previously detected by different methods variety In abundances of these peptides (Pea-CAH-I is more than twice as abundant as Pea-CAH-ll, 12) (see also Note 9 on relative quantification).

Figure 6.6 Near-field photopolymerization based on the resonant excitation of the dipolar LSPR mode of silver nanoparticles, (a) Topographic AFM image of silver nanoparticles before photoexcitation, (b) Close-up image of nanoparticle selected in panel a. (c) Close-up topographic image of the same silver nanoparticle after photopolymerization. (d) Differential image of panels c and d. (e) Spatial map of near-field intensity calculated around a 60-nm silver nanoparticle using the FDTD method. Reprinted with permission from Ref [56]. Copyright (2010) American Chemical Society.

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