4’6 diamino 2-phenylindole DAPI

The presence of PolyPs in these granules is indicated by the following indirect observations. First, volutin granules undergo metachromatic staining by basic dyes, 4,6 -diamino-2-phenylindole (DAPI) fluorescence shifts, and other reactions specific for PolyPs (see... 

The more sensitive and convenient method of PolyP detection in situ is fluorescence microscopy using fluorochromes of the type 4/,6/-diamino-2-phenylindole.2HCl (DAPI), which is commonly used for DNA detection. At a high concentration (50 mg ml 1), it also stains PolyP granules and lipid inclusions (Allan and Miller, 1980 Tijssen et al, 1982 Streichan et al, 1990). DAPI-DNA fluorescence is blue-white, while DAPI-PolyP and DAPI-lipid fluorescence is yellow. The lipid fluorescence is weak and fades in a few seconds, while the PolyP granules appear bright yellow, thus allowing discrimination of the above types of cell inclusions (Streichan et al, 1990). 

Figure 2.4 Fluorescence spectra of DAPI (4, 6 -diamino-2-phenylindole) in 3 mM tris-maleate, pH 5.0 (a) 0.2 /rgml-1 DAPI (b) 0.2 //g ml 1 DAPI plus 10 //g ml 1 PolyP2oo (Tijssen et al., 1982).

DAPI (46-diamino-2-phenylindole) 23, 24, 53,57 degradosome 107, 108, 121 diadenosine tetraphosphate 117 diadenosine-5, 5 "-P1, P4 tetraphosphate... 

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