Determination of DNA Lesions by Liquid Chromatography

Electrochemical detection is also the method of choice for the reducing dCyd-derived products 50HdCyd and 50HdUrd that can be released enzymatically from oxidized DNA (Wagner et al. 2004). After water elimination, the Ug may also be determined by this technique. 

The sugar lesions FUR and 5-MF (Joshi and Ganesh 1994), as well as DNA-ad-ducts (Park et al. 1989) have also be detected with the help of HPLC. 

For optical detection, the product to be determined must have a reasonable absorption coefficient. Saturation ofthe pyrimidine C(5)-C(6) bond destroys the chromophore, and for the determination of Tg yields excreted into the urine, this product was reduced with HI into Thy prior to an HPLC analysis (Cathcart et al. 1984). 

The combination of HPLC and MS (cf. Frelon et al. 2000) is expected to become the method of choice in many cases. The state of the art has been reviewed (Cadet et al. 2002). 

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