Detergent solubilization, liposome preparation

Drug is soluble in neither oil nor water however, it can be retained at the interface of an emulsion. Thus, if a liposomal preparation can be made in which the drug resides in the lipid bilayer, or if it can be solubilized into micelles by an appropriate detergent, an emulsion can probably be made wherein the drug resides at the interface. 

To prepare DNA-loaded liposomes, plasmid pEScFv 2G42D7 is added to OG-solubilized liposomal lipids before final detergent removal by dialysis (when necessary, together with modified AM antibody). The quantity of the plasmid used is 50 pg/mL. Other steps of liposome preparation are described in Subheading 3.1.2. 

Due to their bilayer (phospho-) lipid membrane, the liposomes are often considered biomimetic products, even though the complexity of living cells membranes is hardly achievable by their simple structure, hi order to approach that complexity, several preparation methods are used to tune the hposomes physicochemical and colloidal characteristics (mechanical agitation, solvent evaporation, solvent injection, detergent solubilization, etc.). 

---