Detection system PowerVision

FIGURE 1.11 Schematic of polymer-based detection system. The enzyme-linker antibody has a more compact molecular shape than other polymer carrier-based conjugates and thus allows the attachment of multiple conjugates in close proximity to each other. The abundant conjugated enzymatic molecules are deposited in each antigenic location, which resembles a big-city skyline. From Shi S-R, Quo j, Cote R], et al. Sensitivity and detection efficiency of a novei two-step detection system (PowerVision) for immunohistochemistry. Appi immunohistochem Moi Morphoi. 1999 7 20i-208. 

Adapted from Shi S-R, Guo j, Cote Rj, et al. Sensitivity and detection efficiency of a novel two-step detection system (PowerVision) for immunohistochemistry. AppI Immunohistochem Mol Morphology. 1999 7 201 -208. 

A comparative study by Shi and colleagues compared the PowerVision system with three multi-step detection systems available at that time the ChemMate, ESAB2, and SuperSensitive kits. Three sets of experiments were performed to compare the different methods for immunohistochemical staining of routine FFPE tissue sections. In all three experiment sets, staining was performed under identical conditions, including the use of an optimized microwave AR technique. In these experiments, the polymer-based method outperformed other techniques in all aspects. The sections that were stained using PowerVision and an optimized AR protocol could be satisfactorily restored this was not possible with the other three detection systems. 

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