Derivatized coenzyme

Membrane retention Advantageous for allosteric enzymes. The enzyme and the derivatized coenzyme are retained by the membrane. Drawback problems at high shear rate. 38... 

Type 2 MTs, also currently understood to function exclusively as OMTs, are found in all lignin-producing plants. This family is specific for coenzyme A derivatized phenylpropanoid compounds and appears to be less diverse in sequence and gene number than the type 1 family of MTs (Fig. 2.3). Based on database searches, type 2 OMTs consist of caffeoyl and feruloyl coenzyme-A specific OMTs (CCoAOMTs). The most closely related enzymes to plant CCoAOMTs are the mammalian small molecule methyltransferases, including catechol OMT and bacterial MTs involved in macrolide biosynthesis. 

Scheme 11. Idealized sketch showing the electroen matic oxidation of L-lactate at gold modified electrode surfaces, (a) Lactate dehydrogenase bound to CB-terminated alkylthiol SAMs prepared by covalent attachment of CB to 3-mercaptopropionic acid SAM derivatized with 1,4-diaminobutane. The electroenzymatic oxidation of lactate is observed only in the presence of soluble coenzyme (NAD" ") and a redox mediator (phenazine methosulfate) [215]. (b) Lactate deh3tdrogenase bound to NAD-terminated alkylthiol SAMs prepared by covalent attachment of Af -(2-aminoethyl)-NAD to a cystamine SAM derivatized with pjrrroloquinoline quinone. The reconstituted enzyme is electrically wired to the electrode surface via two NAD" -binding pockets involved in the affinity-binding surface reaction [242].

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