Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Derivatization increasing benzoyl chloride

In analytical LC there are two primary reasons why chemical derivatization of the sample constituents would be necessary, and they are 1) to enhance the separation and 2) to increase the sensitivity of detection. Under certain circumstances, derivatization can also be used to reduce peak asymmetry, i.e. to reduce tailing, or to improve the stability of labile components so that they do not re-arrange or decompose during the chromatographic process. However, sensitivity enhancement is the most common goal of derivatization. For example, aliphatic alcohols that contain no UV chromaphore can be reacted with benzoyl chloride to form a benzoic ester. [Pg.237]

Creasy (68) investigated postcolumn derivatization of A-ethyldiethanolamine and triethanolamine with benzoyl chloride in combination with APCI. Derivatization of one hydroxyl group occurred after mixing the reagent with LC eluent, and sensitivity was not affected by derivatization. A possible advantage of this method is to increase the molecular mass to remove the analyte from interferences. [Pg.309]

Chemical derivatization has become a very popular technique for increasing the sensitivity of a specific type of detector to compounds for which it normally exhibits little or no response. An examples of this procedure would be the reaction of an aliphatic alcohol, which contains no UV chromophore, with benzoyl chloride to form the benzyl ester which could then be detected by the UV detector. Derivatization can also be used to permit the use of an alternative type of detector to increase sensitivity. For example an amino acid may exhibit only weak absorption in the UV, but when reacted with a suitable fluorescing reagent, could be detected by means of the fluorescence detector at concentration levels one or two orders of magnitude lower than with the UV detector. It is clear that derivatization procedures can increase significantly the versatility of many detectors. [Pg.160]

Nitrobenzoylation has been used as a derivatization method by some in preference to benzoylation, since the incorporation of the additional chromophoric group produces a 10-fold increase in the sensitivity of UV detection. The derivatizing agent is 4-nitrobenzoyl chloride in pyridine, and the derivatives are detected by UV photometry at 260 nm. [Pg.425]


See other pages where Derivatization increasing benzoyl chloride is mentioned: [Pg.166]    [Pg.168]    [Pg.6141]   
See also in sourсe #XX -- [ Pg.70 ]




SEARCH



Benzoyl chloride

Benzoyl chloride, benzoylation

© 2024 chempedia.info