2-Deoxyribose, interference with sialic acid

Other methods of removing interference include the use of glucose to suppress deoxyribose pigment formation (Smith et al. 1975). The suppression could be augmented with NaCl and trichloracetic acid and deoxyribose interference at concentrations up to 75 (lM completely eliminated. However, the method leads to decreases (up to 25%) in sialic acid reactivity. 

An automated assay based on the Aminoff method, including acidic butan-l-ol and combining the sensitivity of the assay with ion-exchange purification, has been put forward by Krantz and Lee (1975) and remains the most sensitive of all automated methods. It can detect 1.5 nmoles of sialic acid ( 0.5 xg), eliminates major interference due to 2-deoxyribose and precludes any corrections for the presence of such interference. 

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