12- deoxyphorbol acetate

Tigliane (G5 C7 C61 C3) diterpenes include the highly irritant, toxic, co-carcinogenic, PKC activating phorbol esters from Euphorbiaceae plants. While not being activated by the parent compound phorbol, PKC is activated by plant-derived esters of phorbol, 4-deoxyphor-bol and 12-deoxyphorbol (e.g. 12-0-palmitoyl-16-hydroxy-phorbol 13-acetate, 12-deoxyphorbol 13-benzoate, 12-deoxyphorbol 13-phenylacetate, 12-deoxyphorbol 13-phenylacetate-20-acetate, sapintoxin A (4-deoxyphorbol 12-(2-methylamino)benzoate-l3-acetate) and 12-tetradecanoylphorbol 13-acetate (TPA)), as well as by synthetic phorbol esters. 

Deoxyphorbol 13-phenylacetate-20-acetate (phorbol ester tigliane diterpene)... 

Deoxyphorbol-13 -G-phenylacetate-20-acetate = Epidermal growth factor... 

Deoxyphorbol-13-0-phenylacetate-20-acetate is not protein kinase C-P isozyme-selective in vivo (Kiley et al. 1994). Prolonged treatment (>6h) of cultures in down-modulation studies is complicated by the metabolism of 12-deoxyphorbol-13-0-phenylacetate-20-acetate to 12-deoxyphorbol-13-phenylacetate, a compound which activates all protein kinase C isozymes tested in vitro (Ryves et al. 1991). 

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