2-Deoxyecdysone

PE-free callus from Polypodium vulgare was shown to biotransform ecdysone fo 20-hydroxyecdysone, which is the last step in the biosynthetic pathway of the main plant PE. This hydroxylation is catalysed by a cytochrome P450 enzyme which was subsequently purified from that source (Canals et al, 2005). In another study, Reixach et al. (1999) have shown that 25-deoxy-20-hydroxyecdysone was transformed efficiently in both tissues into 20-hydroxyecdysone, but no 25-deoxyecdysteroids such as pterosterone and inokosterone were formed. Likewise, incubation of 2-deoxyecdysone produced exclusively ecdysone and 20E, indicating a high 2-hydroxylase activity in both tissues. 

A detailed survey of the isolation and chemistry of the ecdysones has appeared. The isolation of deoxycrustecdysone (402a), deoxyecdysone (402b), and a-ecdysone (402c) has been reported. The structures of stachysterone A... 

Tsoupras et al. used 31p nmr to detect the presence of phosphate groups in the 22-adenosinemonophosphoric ester of 2-deoxy-ecdysone and 22-phospho-2-deoxyecdysone (34). Quantitative NMR has been used in several studies. Wayne et al. used 51p nmr for quantitative analysis of organophosphorous pesticides (35). Zon et al. used NW to study chemical and microsomal oxidation of cyclophosphamide ( ), and to determine the half-life of a cyclophosphamide analogue (37). 

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