Deoxy sugar biosynthesis, reaction

Detailed Reaction Mechanism of Deoxy Sugar Biosynthesis... 

The first two enzymatic steps are common to the biosynthesis of all 6DOHs and lead to the biosynthesis of the intermediate TDP-4-keto-6-deoxy-D-glucose. These enzymatic reactions have been extensively studied in different sugar biosynthesis pathways, such as tylosin or mithramycin [13]. 

The key intermediates in the biosynthesis of 6-deoxy sugars are the nucleoside 6-deoxyhexosyl-4-ulose diphosphates (7), formed through enzymic reactions catalyzed by NDP-sugar 4,6-dehydratases (EC 4.2.1.45-47) from primary glycosyl nucleotides. These reactions were observed... 

On the basis of these results, the authors16 proposed the reaction sequence shown in Scheme 4 for the biosynthesis of CDP-L-vinelose from CDP-D-glucose. As with L-mycarose, the 6-deoxyhexos-4-ulose derivative (11) is the substrate for the methylation step leading to the CDP-6-deoxy-3-C-methylhexos-4-ulose (12). The corresponding dTDP-sugar might, therefore, be one of the unidentified reaction-products in the biosynthesis of dTDP-L-mycarose. 

Figure 1.62 Biosynthesis of NDP sugars from sugar 1-phosphate and reaction originating from NDP-4-keto-6-deoxy-D-sugars.

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