14-deoxy -20-hydroxyecdysone activity

PE-free callus from Polypodium vulgare was shown to biotransform ecdysone fo 20-hydroxyecdysone, which is the last step in the biosynthetic pathway of the main plant PE. This hydroxylation is catalysed by a cytochrome P450 enzyme which was subsequently purified from that source (Canals et al, 2005). In another study, Reixach et al. (1999) have shown that 25-deoxy-20-hydroxyecdysone was transformed efficiently in both tissues into 20-hydroxyecdysone, but no 25-deoxyecdysteroids such as pterosterone and inokosterone were formed. Likewise, incubation of 2-deoxyecdysone produced exclusively ecdysone and 20E, indicating a high 2-hydroxylase activity in both tissues. 

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