Dendrimers sensitization process

Vogtle F, Gestermann S, Kauffmann C et al (1999) Polypropylene amine) dendrimers with peripheral dansyl units protonation, absorption spectra, photophysical properties, intraden-drimer quenching, and sensitization processes. J Am Chem Soc 121 12161-12166... 

In both cases, the luminescence of the species A is quenched, and in the case of energy transfer, it can be replaced by the luminescence of species B (sensitization process). In dendrimers, energy- and electron-transfer processes can also occur among nearby molecular components incorporated in the dendrimer structure. For example, in a dendrimer containing A and B component units, excitation of A may be followed by energy (Eq. 10) or electron (Eqs. 11 and 12) transfer to B (5) (Fig. 3b) ... 

Furthermore, upon encapsulation and formation of dendrimer-encapsulated dyes, new fluorescence properties which were different from each constituent were observed and assigned to quenching/sensitization processes. The fluorescence of peripheral dansyl groups of the dendrimer was totally quenched through energy transfer by the encapsulated dyes while hosted dyes experienced partial quenching. 

The general method utilized to prepare E5-Ab solutions obviates the need for stocking large numbers of reagents which would be necessary if different activation methods were used for each antibody. A number of specific antibodies immobilized by this process have shown response similar to that of the same antibodies when adsorbed as immune complexes in the Stratus system. In addition, the dendrimer-coupled antibodies have shown dramatic improvements in sensitivity, flexibility and precision for the enzyme immunoassay system. Feasibility demonstration of an assay for DNA probes is a prelude to what can possibly be achieved with these dendrimer-based reagents. 

A number of reviews have appeared of topical interest. These include photoisomerism of azo dyes, ° photofunctional polysilanes,photochromic pigments, rare earth complexes, pressure sensitive paints, electron-transfer processes,electroactive dendrimers, chiral polyisocyanates, photodefin-able benzocyclobutene, photoconductive polymers,excited states in conjugated polyenes, photosensitive materials and polyazomethanes. ... 

---