Delphinidin-3-rutinoside-7 -glucosid

The effects of pH under model conditions (0.6 to 5.5 for 24 hr) were covered by Nielsen et al. for four anthocyanins (3-O-glucoside, glycosylated cyanidin, rutinoside, and delphinidin rutinoside). After 24 hr, over 90% of the anthocyanins were intact up to pH 3.3, while instabihty greatly increased at pH greater than 4.5. 

Fig. 16 RP-HPLC separation of black currant juice. Peaks 1 = delphinidin 3-glucoside 2 = delphinidin 3-rutinoside 3 = cyanidin 3-glucoside 4 = cyanidin 3-rutinoside 5 = pelargonidin 3-glucoside (= int. std.) 6 = pelargonidin 3-rutinoside. (From Ref. 157.)...

Figure 9 TLC separation on cellulose (0.1 mm, Merck) of anthocyanins from black currant (Ribes nigrum) with densitometric detection at 52S nm using cone, hydrochloric acid-formic acid-water (25 24 51) as mobile phase. Peak identities (1) Cyanidin-3-rutinoside (2) >elphinidin-3-nitinoside (3) Cyanidin-3-glucoside (4) Delphinidin-3-glucoside.

Tatsuzawa, F. et al., Acylated delphinidin 3-rutinoside-5-glucosides in the flowers of Petunia reitzii. Phytochemistry, 54, 913, 2000. 

Delphinidin-3-rutinoside, Cyanidin-3-rutinoside, Cyanidin-3-glucoside Black currant Rat, Human, Rabbit 16, 58, 59... 

Quercetin, isorhamnetin, quercitrin, kaempferol 3-O-rutinoside, isorhamnetin 3-O-glucoside, and morin Betulinic acid, oleanolic add, and ursolic add Vanillic acid, caffeic add, p-coumaric add, ferulic acid, delphinidin, cyanidin, quercetin, kaempferol, isorhamnetin, procyanidin B2, and procyanidin B3... 

Anthocyanins and anthocyanids are usually separated on microcrystalline cellulose. Andersen and Francis (1985) were able to separate flavonoids on cellulose in the mobile-phase HCl-formic acid-water (24.9 23.7 51.4). The Rf values obtained were 0.03 for delphinidin, 0.06 for cyanidin, 0.11 for pelargonidin, 0.26 for cyanidin 3-glucoside, 0.48 for cyanidin 3-rutinoside, and 0.95 for pelar-goniden 3-sophoroside-5-glucoside. 

The fruits of Cornus alternifolia showed similar anthocyanin profile as those of Cornus controversa [Vareed et al., 2006], the relative amounts of anthocyanins in these fruits were found in the following order delphinidin-3-O-rutinoside > delphinidin-3-O-glucoside cyanidin-3-O-glucoside. The anthocyanin content in Cornus alternifolia fruits was several times higher than in other commonly consumed fiuits. 

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