Dehydrogenases, potential industrial

The electrochemical sensing of NADH is of great interest in the development of a dehydrogenase-based amperometric biosensor owing to the ubiquitous use of NADH as a cofactor for over 300 enzymes and in the fine chemicals industry using NAD -dependent biocatalysts [106]. The oxidation of NADH at bare and modified electrodes has been well studied and the oxidation process is dependent on the nature of the electrode used. The direct electrochemical oxidation of NADH at the bare electrode, irrespective of its nature, requires a high overpotential, despite the formal potential of the NAD /NADH redox couple at pH 7, which is reported to be... 

The potential applications of oxidoreductases in industry are numerous. Alcohol dehydrogenases are useful in stereospecific synthesis and production of high-cost compounds such as cyclic ethers (see below), and have also been used in the production of flavour aldehydes e.g. geraniol [94]. With mesophilic enzymes, however, limitations due to narrow specificity, instability to heat and organic solvents and loss of activity on immobilization have been incurred. The use of these enzymes from thermophilic sources in inunobilized and continuous reactor systems (including biosensors) has also been proposed for the regeneration of... 

---