Degradation of oligosaccharides coupled with mass spectrometry

3 Degradation of Oligosaccharides Coupled with Mass Spectrometry 

There are a variety of chemical or enzymatic degradation methods of oligosaccharides that have been used in conjunction with mass spectrometry. [246,247] Three of these methods are described in detail below. 

Chromium oxide action on the oc- and (3-anomers of the glycosidic bonds. 

GlcNAc/31 -4Man/31 -4GlcNAc/31 -4 GlcNAo Gal/31-4Glc/31-2Manal 3 

The MALDI spectrum from successive enzymatic digests of a native oligosaccharide. The structural information obtained from the specificity of the exoglucosidases used is indicated by italics in the complete structure in A. Reproduced (modified) from Kuster B., Naven T.J.R and Harvey D.J., J. Mass Spectrom., 31, 1131-1140,1996, with permission. 

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