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DeCyder software

Eight significantly regulated proteins are indicated by arrows and respective spot number. In the zoomed sections two differentially regulated proteins white box) are displayed in 3D view obtained with the DeCyder software. [Pg.47]

Erom the DeCyder software it is possible to find the background-subtracted spot volumes, i.e., for a single spot the lowest lOfh percentile pixel values on the spot boundary have been excluded. Several features of this raw data require calibration and normalization prior to statistical analysis. The volume values are affected by dye-specific system gains and constant additive biases. In figure 3.10 the Cy5 and Cy3 spot volumes of one gel are plotted against each other. It can clearly be seen that the difference between Cy5 and Cy3 spot volumes increases when the spot volumes increase. [Pg.49]


See other pages where DeCyder software is mentioned: [Pg.149]    [Pg.41]    [Pg.42]    [Pg.42]    [Pg.43]    [Pg.149]    [Pg.41]    [Pg.42]    [Pg.42]    [Pg.43]    [Pg.145]    [Pg.336]    [Pg.38]    [Pg.38]    [Pg.271]    [Pg.7]    [Pg.12]    [Pg.13]   
See also in sourсe #XX -- [ Pg.42 ]

See also in sourсe #XX -- [ Pg.42 ]




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DeCyder

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