Spectrum deconvolution of complex

Numerous, wide-ranging spectroscopic techniques will be presented in this volume, with the exception of nuclear magnetic resonance (NMR), which was the subject of Volumes 176, 177, and 239 of Methods in Enzy-mology, and mass spectrometry, which was the subject of Volume 193. Examples of techniques from each of three major areas, ultraviolet/visible spectroscopy, vibrational spectroscopy, and electron or electron/nuclear magnetic resonance, are presented in this volume. Also included are special topics like rapid-scan diode-array spectroscopy, terbium labeling of chromopeptides, and deconvolution of complex spectra that are covered in chapters in Section IV of this volume. 

Much work has been done on P NMR of membranes in microorganisms. The future holds the possibility of similar detailed studies on multicellular systems and animals. We shall probably see more use of H- P cross-polarization, two-dimensional, and other techniques for better deconvolution of complex spectra, as well as a better understanding of the relaxation behavior. The spectra still contain more information than we can conveniently extract from them. 

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