Deconvolution Methods for Solid-Phase Pool Libraries

3 DECONVOLUTION METHODS FOR SOLID-PHASE POOL LIBRARIES 

While the structure determination methods seen in the previous section are based on the analytical identification of the positive library individuals, other methods exist to perform the so-called deconvolution of the library complexity and allow the eventual identification of one or more positives from the library without determining their structure by analytical methods. The most common approach is based on iterative synthesis cycles of less complex pools deriving from the original active pool(s) until single compounds are prepared and tested iterative deconvolution) (3). 

Iterative deconvolution selects the pools solely according to the screening results, and no analytical characterizations are performed at any deconvolution stage. This is particularly useful when sophisticated analytical instrumentation is not available. The measured activity is always the sum of each individual contribution. Assuming that the SP assessment studies, the monomer rehearsal, and the model library synthesis 

625-member SP pool library M4 determined, M -M3 randomized 5 pools, 125 components/pool 20 reactions to prepare it 

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