Decalcifying agents

As soon as possible after removal from the uterus, the fetuses are euthanized and fixed in Bouin s solution. Fixation takes 7-14 days (see Note 2). Bouin s is the preferred fixative as it is sufficiently acidic to act as a decalcifying agent, which facilitates sectioning through bone, particularly the skull. Specimens may require hardening, by immersion in IMS for at least 24 hours to aid in sectioning. 

Callis, G., and Sterchi, D. 1998. Decalcification of bone Literature review and practical study of various decalcifying agents, methods, and their effects of bone histology. J. Histotechnol. 27 49-58. 

Use Analytical chemistry (reagent for pentoses and with vanillin for determining the presence of free hydrogen chloride), decalcifying agent for bones, preparation of pharmaceuticals and dyes, resins, preservative for cut flowers, textile dyeing and printing. 

Crystallizes out from water with two molecules of water of crystallization. Anhydrous form melts at 218°C used for testing of lignins in wood, decalcifying agent for bones, pharmaceutical, and dye intermediate, textile dyeing and printing, etc. 

In addition, PPA shows a good chemical resistance. In particular, the resistance to decalcifying agents such as amidosulfonic acid and to hot oils, battery acid, and brake fluid has been pointed out. ... 

Decalcification of Dentine. Fukuda (1966) has studied the spectra of normal human dentine and dentine which had been decalcified by treatment with live different agents EDTA, nitric acid, sulfosalicylic acid, trichloroacetic acid, and lithium lactate. Quick and complete decalcification of dentine was effected by nitric, trichloroacetic, and sulfosalicylic acids, whereas decalcification by EDTA and lithium lactate was slower and less complete, the PO4 bands at 1033 and 1095 cm" were suggested as bands to be examined for decalcification (Fig. 19.15). 

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