Death image

Figure 5.1. Cellular apoptosis. Various critical cellular apoptotic integrated processes are schematically shown. From membrane, to mitochondria, and caspase cascades, and the nucleus all become involved. The ceU is blebbing at bottom right in the process of programmed cell death. (Image acquired with permission from from BioOncology of Genentech Corporation (www.biooncology.com.) See insert for color representation of this figure.

Fig. 1.1 John Osborn (1939-2000) with his students at Harvard in 1971. From left. A1 Kramer, Sue Bezman, Steve Wilson, the author, John Shapley, Dave Rice kneeling), Dick Schrock. Osborn left Harvard (putting him in good company see Chap. 4) for Strasbourg in 1975, where he continued his outstanding work in organotransition metal chemistry until his untimely death (Image courtesy of John Shapley)...

Technical language and jargon are useful as professional shorthand, but they are barriers to successful communication with the public. Use simple, nontechnical language and vivid, concrete images that communicate on a personal level. Avoid distant, abstract, unfeeling language about deaths, injuries, and illnesses. 

Kuimova MK, Botchway SW, Parker AW, Balaz M, Collins HA, Anderson HL, Suhling K, Ogilby PR (2009) Imaging intracellular viscosity of a single cell during photoinduced cell death. Nat Chem l(l) 69-73... 

Various antibody preparations have been developed that facilitate imaging of vascular-related conditions, including myocardial infarction, deep vein thrombosis and atherosclerosis. Anti-myosin monoclonal antibody fragments (Fab) labelled with mIn, for example, have been used for imaging purposes in conjunction with a planar gamma camera. The antibody displays specificity for intracellular cardiac myosin, which is exposed only upon death of heart muscle tissue induced by a myocardial infarction (heart attack). 

The image of death presented in stanza 1 is b. views Death as an intruder. 

Based on the qualitative and quantitative analyses of a two-dimensional fluorescent image scan using a low laser power, areas within the field can be chosen for rescanning at a higher killing laser power. This results in the selective cell death of those cells that meet a specific fluorescence-labeling criteria, i.e., selecting for those cells that are either above or below a certain fluorescence-intensity threshold value (see Fig. 5). 

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