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De novo design systems

Furthermore, even if the biological receptor is known and structurally determined, modern techniques such as virtual screening and docking experiments or de novo design systems have to take into account several alternative conformations of the small molecules under investigation to estimate and rank different binding modes and constants with locally optimized electrostatic and steric interactions between the ligand and its receptor [8]. [Pg.153]

In contrast to the use of self-assembly reactions and metal ion coordination preferences to direct the construction of mixed cofactor systems, the use of SPPS or selective chemical ligation allows for the direct covalent attachment of cofactors for the construction of mixed cofactor systems within de novo design. Figure 11 shows the flavocy-tochrome maquette constructed by Dutton and co-workers (149) using a flavin moiety covalently attached to a unique cysteine residue inside a four helix bundle with bis-histidine binding sites for heme... [Pg.431]

Ryadnov MG, Ceyhan B, Niemeyer CM, Woolfson DN. Belt and braces a peptide-based linker system of de novo design. J Am Chem Soc 2003 125 9388-9394. [Pg.391]

A major advancement derived from research into homo- versus heterodimerization specificity includes the use of de novo designed coiled coils for applications that require selective heterodimerization, such as was described by Tripet et al., 67 who developed a unique affinity matrix protein tag system as a rapid and sensitive method to detect, purify, and characterize newly expressed recombinant peptides and proteins from cell extracts. In... [Pg.73]

Therefore, de novo designed two-stranded parallel and antiparallel coiled coils become ideal model systems to study the factors that contribute to our understanding of protein folding and assembly. [Pg.89]

From Table 1 it can be seen that if the size of the i, i+4 lactam is decreased by the incorporation of Asp in place of Glu [DK (i, i+4)], a peptide with only 22% helical structure results under benign conditions and 34% in 50% TFE, compared to 99 and 105%, respectively, for its lactam counterpart, 2EK (i, i+4). Note that it also exhibits less helicity than its linear counterpart, linear 2DK, with values of 27 and 83%, respectively, in the absence and presence of 50% TFE. Clearly, in this de novo designed model system, DK lactams result in peptides with destabilized helices. [Pg.97]

The following new trends in enzymatic synthesis can be delineated the development of new enzymatic reactions enzyme immobilization and stabilization the use of organic solvents and two phase systems site-directed mutagenesis chemical modification of enzymes antibody catalysis catalysis by RNA and DNA de novo design ofbiocatalists employment of recombinant DNA for production of enzymes and use computational and combinatorial methods... [Pg.168]

A major addition to the second edition is Chapter 9, which discusses computational enzymology. This chapter extends the coverage of quantum chemistry to a sister of organic chemistry—biochemistry. Since computational biochemistry truly deserves its own entire book, this chapter presents a flavor of how computational quantum chemical techniques can be applied to biochemical systems. This chapter presents a few examples of how QM/MM has been applied to understand the nature of enzyme catalysis. This chapter concludes with a discussion of de novo design of enzymes, which is a research area that is just becoming feasible, and one that will surely continue to develop and excite a broad range of chemists for years to come. [Pg.631]


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See also in sourсe #XX -- [ Pg.2 , Pg.1125 ]




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