Data matrices capillary electrophoresis

Pharmacokinetic data analysis requires determination of the analyte in various body fluids. In the case of therapeutic antibodies, serum is the most common matrix to be analyzed. For a critical interpretation of pharmacokinetic data the chosen bioanalytical methods must be considered. The most frequently used for mAbs include enzyme-linked immunosorbent assay (ELISA), capillary electrophoresis (CE)/polyacrylamide gel electrophoresis (PAGE), fluorescence-activated cell sorting (FACS), and surface plasmon resonance (SPR). The challenges and limitations of bioanalytical methods used for the analysis of mAb concentrations are discussed in detail in Chapter 6. 

Today s instruments of electrophoresis often incorporate capillary tubes to hold the sequence gel, automatic sample loading, separation matrix loading and removal, data collection and analysis, and provide significant improvement over the manual gel preparation and lane tracking required in slab-gel sequencers. 

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