Dansyl chloride, sequencing peptides with

The amino-terminal (N-terminal) residue of a protein can be identified by reacting the protein with a compound that forms a stable covalent link with the free a-amino group, prior to hydrolysis with 6 M HC1. The labeled N-terminal amino acid can then be identified by comparison of its chromatographic properties with standard amino acid derivatives. Commonly used reagents for N-terminal analysis are fluorodinitrobenzene and dansyl chloride. If this technique was applied to the oligopeptide above, the N-terminal residue would be identified as Val, but the remainder of the sequence would still be unknown. Further reaction with dansyl chloride would not reveal the next residue in the sequence since the peptide is totally degraded in the acid hydrolysis step. 

Peptide analysis, sequencing and synthesis All fluorescent dye labels with reactive groups for amine, dansyl chloride, dabsyl chloride. Fluorescent isothiocyanates. N-Terminal amino acid analysis, peptide sequencing, peptide synthesis, labeling peptides in solution, solid-phase synthesis of labeled peptides 37... 

---