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D-Mannanases

Flavobacterium dormitator growing on a D-mannan synthesized an exo-a-l,2-D-mannanase the purified enzyme (mol. wt. 9 x 10 , pH optimum 7.0, Km for yeast mannan 0.16% w/v) was stable at pH 6.0, was not activated by Zn + ions, and was highly specific for the a-(l - 2)-linkages in yeast mannan.  [Pg.394]


D-Mannose to D-Galactose Ratio151 of Residual Nondialyzable Fractions after Treatment with Bacillus subtilis /S-D-Mannanase... [Pg.273]

Progressive Action of /3-D-Mannanase on Locust-bean Gum119... [Pg.273]

Galactomannans and oligosaccharide fragments are susceptible to hydrolysis by a number of enzymes, including a-D-galactosidase90 (EC 3.2.1.22), /S-D-mannanase, exo-/3-D-mannanase,91 /3-D-mannosidase, and exo-/3-D-mannan mannobiohydrolase (EC 3.2.1.100).92 Early applications of enzymic... [Pg.165]

A way of reconciling the observed proportions of mannobi-, tri-, and tetra-oses and heterosaccharides,16113 released by guar-seed jS-D-mannanase (from hot-water-soluble carob galactomannan), with this model, would be if the enzyme preferentially hydrolyzes appropriate, unsubstituted d-mannosyl residues (as in 31) then, it would only be able to hydrolyze on the reducing side of a substituted D-mannose (as in 32) if there were a... [Pg.178]

The structures of the cell-wall D-mannans of several other yeasts have been investigated381 by use of this exo-a-D-mannanase. Five were degraded to the (l->6)-a-D-mannan chain. Those which contained jB-linked d-mannosyl units or a-D-galactosyl groups in the side chains were not significantly hydrolyzed. However, removal, by partial hydrolysis with acid, of the a-D-galactosyl units from five galactomannans, and of /3-linked D-mannosyl units from three other D-mannans, rendered these polysaccharides partially susceptible to hydrolysis by exo-a-D-mannanase, consistent with an a-linked-D-mannan structure. [Pg.248]

Takahashi, R., Mizumoto, K., Tajika, K., and Takano, R., Production of oligosaccharides from hemicellulose of woody biomass by enzymatic-hydrolysis. 1. A simple method for isolating beta-D-mannanase-producing microorganisms. Mokuzai Gakkaishi 1992, 38 (12), 1126-1135. [Pg.1535]


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Mannanase

Mannanases

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