D-iso-Lysergic Acid Amide

A solid residue of 3.45 g. comprising the normal and iso , forms of d-lysergic acid N,N-diethyl amide is obtained. This material is dissolved in 160 mL. of a 3-to-l mixture of benzene and chloroform, and is chromatographed over 240 g. of basic alumina. As the chromatogram is developed with the same solvent, two blue fluorescing zones appear on the alumina column. The more rapidly moving zone is d-lysergic acid N,N-diethyl amide which is eluted with about 3000 mL. of the same... 

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