Cytokine bead array

Key words T cells, PBMC, NFAT/AP-1 assay, Phosphotyrosine blot. Calcium flux. Cytokine bead array (CBA), H-thymidine incorporation, siRNA, CD25, CD69, TCR, PTP, Small molecule inhibitors... 

McDuffie, E., et al. (2006) Detection of cytokine protein expression in mouse lung homogenates using suspension bead array. J Inflamm (Land). 3, 15. 

Fig. 11.15. A multiplex bead array that can be used to capture multiple soluble analytes. In the dot plot, 15 types of beads are distinguished by their red/orange ratios they capture 15 different cytokines. For example, the beads in Region 1 capture interleukin (IL)-9 in Region 5, IL-2 in Region 9, IL-5 and in Region 13, MCP-1. The green fluorescence histograms from each region show, by their intensity, how much cytokine has been captured by each type of bead. Standard curves can relate the green fluorescence intensity to the concentration of cytokine in the sample. The experimental format illustrated in the cartoon here is patterned directly from work by RT Carson and DAA Vignali (1999).

Fig. 12.17 Inhibition of phosphorylation of p38 MAP kinase in neutrophils (a, flow cytometry) and cytokine production (c-e, flow-cytometric bead array) in whole human blood stimulated with 100 ng/mL LPS, with either DS-96 or PMB (b) Forward scatter/side scatter profile and the gating for p38 MAPK-negative and positive gates obtained on unstimulated (negative control) and LPS-stimulated (positive control) cells, respectively...

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