Cytochrome, rapid flow techniques

The reaction of 02 with cytochrome c oxidase to form the oxygenated species A (Fig. 18-11) is very rapid, occurring with apparent lifetime T (Eq. 9-5) of -8-10 is.139 Study of such rapid reactions has depended upon a flow-flash technique developed by Greenwood and Gibson.136/140/141 Fully reduced cytochrome oxidase is allowed to react with carbon monoxide, which binds to the iron in cytochrome a3 just as does 02. In fact, it was the spectroscopic observation that only half of the... 

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