Cytochrome electrochemical conversion

Because LCEC had its initial impact in neurochemical analysis, it is not, surprising that many of the early enzyme-linked electrochemical methods are of neurologically important enzymes. Many of the enzymes involved in catecholamine metabolism have been determined by electrochemical means. Phenylalanine hydroxylase activity has been determined by el trochemicaUy monitoring the conversion of tetrahydro-biopterin to dihydrobiopterin Another monooxygenase, tyrosine hydroxylase, has been determined by detecting the DOPA produced by the enzymatic reaction Formation of DOPA has also been monitored electrochemically to determine the activity of L-aromatic amino acid decarboxylase Other enzymes involved in catecholamine metabolism which have been determined electrochemically include dopamine-p-hydroxylase phenylethanolamine-N-methyltransferase and catechol-O-methyltransferase . Electrochemical detection of DOPA has also been used to determine the activity of y-glutamyltranspeptidase The cytochrome P-450 enzyme system has been studied by observing the conversion of benzene to phenol and subsequently to hydroquinone and catechol... 

Proton-coupled electron transfer (PCET) reactions play a vital role in a wide range of chemical and biological processes. For example, PCET is required for the conversion of energy in photosynthesis [1] and respiration [2], In particular, the coupling between proton motion and electron transfer is involved in the pumping of protons across biological membranes in photosynthetic reaction centers [1] and in the conduction of electrons in cytochrome c [3]. In addition to biological processes, PCET is also important in electrochemical processes [4, 5] and in solid state materials [6]. 

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