Cytidine monophosphate incorporation

FIGURE 2.3 Representativemassspectrafortheieactionofdeprotonated2-deoxy-5-cytidine monophosphate (5-dCMP) with D2O in a quadrupole-ion trap. Reaction times are (a) 0 ms, (b) 250 ms, (c) 2000 ms, and (d) 10,000 ms. Peaks are labeled as D , where n equals the number of incorporated denterinm atoms. (Reproduced from Chipuk, J.E. Brodbelt, J.S. J. Am. Soc. Mass Spectrom. 2007,18, 724-736. With permission from Elsevier.)... 

P33 Popov, N., Schmidt, S., Schulzeck, S. and Matthies, H. Incorporation of intraventricularly administered (3H)-uridine monophosphate, pH] cytidine monophosphate, and (14C)-orotic acid into brain and liver RNA of the rat. Acta Biol. Med. Ger., 28, 13-20 (1972) (Germ.)... 

It had previously been shown by a number of workers, and in a number of systems (138, 809-818) that the AMP moiety of labeled ATP was incorporated into soluble RNA, and that this incorporation occurred predominantly in a terminal position (as shown by recovery of the label, after alkaline hydrolysis of the RNA, as adenosine rather than as 2 - and 3 -AMP). Hecht and co-workers (193,813,814) showed that the incorporation of ATP was very small when preincubated RNA was used, but could be restored completely by either prior or simultaneous addition of C3rtidine triphosphate (CTP). This suggested that the terminal AMP had to be attached to a cytidine monophosphate (CMP) end group. This was further corroborated by the fact that in the absence of ATP, but not in its presence, most of the incorporated CTP was, itself, terminal. For every mole of ATP incorporated, there seemed to be two moles of CTP taken up. The terminal sequence of sRNA, therefore, emerged as adenylyl-5 3 -cytidylyl-5 -3 -cytidylyl-5 -3 -RNA (RNApCpCpA) and that the presence of this end group was, indeed, necessary for attachment of amino acids was shown by further detailed experiments of Hecht et al. (188). 

Using a nonequilibrium PCM that incorporates electronic reorganization using CMP, cytidine monophosphate dTMP, deoxythymidine monophosphate. 

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