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Cysteine-containing fusion tag

Cysteine-Containing Fusion Tag for Site-Specific Conjugation of Therapeutic and Imaging Agents to Targeting Proteins... [Pg.275]

We have recently developed a cysteine-containing fusion tag for site-specific conjugation (Fig. IB, also in ref. 1). This tag, named Cys-tag, is an a-helical... [Pg.276]

Figure 17.27 The EPL process involves a fusion protein containing an intein tag plus a CBD. The fusion protein is captured on an immobilized chitin resin and after removal of contaminating proteins, it is eluted using thiophenol, which cleaves at the thioester bond between the intein and the desired expressed protein. This releases a phenylth-ioester-activated protein that can be used in the native chemical ligation reaction with another peptide containing an N-terminal cysteine residue. Conjugation results in a native amide (peptide) bond formed between them. Figure 17.27 The EPL process involves a fusion protein containing an intein tag plus a CBD. The fusion protein is captured on an immobilized chitin resin and after removal of contaminating proteins, it is eluted using thiophenol, which cleaves at the thioester bond between the intein and the desired expressed protein. This releases a phenylth-ioester-activated protein that can be used in the native chemical ligation reaction with another peptide containing an N-terminal cysteine residue. Conjugation results in a native amide (peptide) bond formed between them.
See other pages where Cysteine-containing fusion tag is mentioned: [Pg.275]    [Pg.266]    [Pg.275]    [Pg.266]    [Pg.13]    [Pg.337]    [Pg.497]    [Pg.337]   


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