Cyclodextrin adsorption, chromatographic

Since the chloroanilines are sufficiently retained (k >5) in a 10 % v/v methanol water eluent, and the Ibuprofen enantiomers are sufficiently retained in a 30 % v/v acetonitrile buffer eluent, these solvents were selected as carrier solvents for the displacement chromatographic separations. Also, these solvents were used to determine the adsorption isotherms of p-nitrophenol and 4-t-butylcyclohexanol on beta-cyclodextrin silica. The isotherms were determined from frontal chromatographic measurements as described in (56). The isotherms are shown in Figs. 7 and 8. Since both isotherms are downwardly convex, p-nitrophenol and 4-t-butylcyclohexanol might prove useful displacers for our test solutes, provided that they are more strongly adsorbed that the solutes. 

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