Cyanoalkyl bonded phase

S S, by vol). After purification of the extract on a Florisil column and on a Sep Pak cartridge, TLC was performed on reversed-phase plates. The plates were developed with dichloromethane methanol (70 30, by vol). The specific type of the reversed-phase material, however, was not mentioned. Recent applications of polar bonded phase TLC plates (e.g. aminoali ] or cyanoalkyl groups bonded to silica) in the vitamin K] field were not found. 

Amlnoalkyl and cyanoalkyl (nitrile) bonded phases (the alkyl group is usually propyl) are moderately polar. The former is particularly useful in separating mixtures of sugars and other carbohydrates (Fig. 2(b)), whilst the latter is used as a substitute for unmodified silica, giving more reproducible retention factors and less tailing, especially with basic solutes. Both normal-phase and reversed-phase chromatography is possible by appropriate choice of eluents. 

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