Microisland cultures

Segal In the temperature-dependent febrile seizures, the seizures tend to occur typically during a rise in temperature. InvitroX have seen a similar effect in solitary neurons in microisland cultures where it is not the absolute temperature but rather the change in temperature that increases the excitability (M. Segal, unpublished results). Could this be the case for some of the muscle disorders ... 

Small circuits of hippocampal neurons grown in microisland cultures displayed both ictal and interictal epileptiform activity. Not only was both ictal and interictal activity seen in circuits with two excitatory neurons, but both types of activity were seen also in two-neuron circuits containing one excitatory neuron and one inhibitory neuron. The activity in these El microislands persisted even when inhibitory transmission was blocked pharmacologically, suggesting that epileptiform activity could be generated under these conditions by the single excitatory neuron. Studies of such sohtary neurons produced the simplest... 

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