Culture-independent methods diversity in microbial communities

3 Culture-independent methods diversity in microbial communities 

In the past few years, approaches for studying microorganisms in food have undoubtedly changed. 

Given the importance of fermented food in human life, much effort has been expended toward describing the microbial populations acting in the different food fermentations. A deeper understanding of microbial ecology can lead to an improvement in the quafity and safety of products. The result has been the development of new techniques that were added to traditional microbial methods based on the cultivation of microorganisms and the resolution of the culture media bias distorting the real picture of microbial communities. 

In recent years, molecular biology has encompassed a range of DNA- and RNA-based technologies, which are revolutionizing the way that microorganisms are studied 

SSCP-PCR No need for gradient gels Dependent on the avail- 

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