CtBP3/BARS

Carcedo, C. H., Bonazzi, M., Spano, S., Turacchio, G., Colanzi, A., Luini, A., and Corda, D. (2004). Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/ BARS. Science 305, 93-96. 

Purification and Functional Properties of the Membrane Fissioning Protein CtBP3/BARS... 

CtBP3/BARS (BARS) is a member of the CtBP family of proteins (Chinnadurai, 2002), and it was initially identified as the 50-kDa substrate of brefeldin A (BFA)-dependent ADP-ribosylation (De Matteis et al., 1994 Di Girolamo et al., 1995). This protein is involved in the disassembly of the Golgi complex induced by BFA, suggesting that it has a role in the maintenance of the structure and/or function of this organelle (Mironov et al, 1997). This was later confirmed as the fissiogenic activity of BARS (Weigert... 

Experimental Approaches to Study CtBP3/BARS Fimction... 

Fig. 5. Subcellular localization of BARS. (A) To examine the localization of the individual CtBPs, COS7 cells were transfected with BARS (CtBP3/BARS), CtBPl, or CtBP2, and 24 h after transfection they were fixed with 4% paraformaldehyde for 10 min, and double stained with 1 g/ml affinity purified SNl antibody (green) and an anti-tubulin antibody (red). (B) (C) COS7 cells were transfected with BARS, and 24 h after the transfection they were fixed with 4% paraformaldehyde (B), or first permeabilized with SLO, incubated at 37° for 5 min, and fixed with 4% paraformaldehyde (C), for 10 min at room temperature. The cells were double-labeled with 1 iig/ml affinity-purified SNl antibody (red) and a monoclonal anti-giantin antibody (green) the merged images are also shown. The Golgi and plasma membrane localization of BARS are seen after SLO-permeabiUzation, which removes the soluble cytosolic pool of BARS.

Spano, S. (2002). Functional and molecular characterization of CtBP3/BARS, a protein involved in the control of the Golgi complex. PhD thesis.. 

Spano S, Hidalgo Carcedo C, Luini A, and Corda D. (2006). CtBP3/BARS and membrane fission. In CtBP Family Proteins (G. Ghinnadurai, Ed.), Georgetown, TX Landes Bioscience. 

---