Crystallization strategies for

It was shown that this remarkable phase behaviour could be understood on the basis of the sensitivity to the form of the pair potential of the phase diagram of small attractive colloidal particles [268-270]. Moreover, it was soon realized that successful protein crystallization depends on the location (protein concentration and temperature) in the phase diagram [271-275]. Control of protein crystal nucleation around the metastable liquid-liquid phase boundary appears key to the development of systematic crystallization strategies (for a concise review see [276]). This phase boundary can be manipulated by depletion interactions through the addition of non-adsorbing polymers such as polyethylene glycol. 

Chayen, N. E. (Ed.). (2007). Protein crystallization strategies for structural genomics. Biotechnology Series. La Jolla International University Line. 

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